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. 2004 Mar 29;164(7):1009–1020. doi: 10.1083/jcb.200310061

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — YSK1 shows activity-dependent targeting to the Golgi apparatus. (A) Bait constructs comprising full-length YSK1, or the indicated deletion constructs were tested against full-length rat GM130 or an empty bait plasmid (control) for the ability to grow on selective medium (QDO), compared with nonselective medium (−LW) in the yeast two-hybrid system. Lighter colony color on QDO is an indicator of a strong interaction. Boxed regions indicate the canonical serine/threonine kinase domain (green), and the extension to this necessary for binding to GM130 (red). (B) HeLa cells transfected with myc-tagged YSK1 constructs for 18 h were costained with the 9E10 mAb to the myc epitope and a sheep antibody to GM130. Bar, 10 μm.