Table I. Yeast strains.
| Strain | Genotypea |
|---|---|
| LHY1101 | pHA-rsp5 C2Δ [TRP1] rsp5Δ::HIS3 trp1 leu2 ura3 lys2 ade2 bar1 or bar1Δ::HIS3 |
| LHY1103 | pHA-RSP5[TRP1] rsp5Δ::HIS3 trp1 leu2 ura3 lys2 ade2 bar1 or bar1Δ::HIS3 |
| LHY2920 | pNotI-RSP5[TRP1] pGFP-CPS[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY2922 | prsp5 C2Δ [TRP1] pGFP-CPS[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY2973 | pNotI-RSP5[TRP1] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY2974 | prsp5 C2Δ [TRP1] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY3454 | pGFP-CPS[URA3] ura3Δ leu2Δ his3Δ met15Δ |
| LHY3834 | prsp5 C2Δ [TRP1] pGFP-PHM5[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY3835 | prsp5 C2Δ [TRP1] pUB-GFP-PHM5[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY3876 | prsp5 5K→Q [TRP1] pGFP-CPS[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY3923 | prsp5 5K→Q [TRP1] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY3924 | pNotI-RSP5[TRP1] ura3::STE2-GFP::URA3 rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY3925 | prsp5 C2Δ [TRP1] ura3::STE2-GFP::URA3 rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4012 | pGFP-RSP5[URA3] vps4Δ::kanMX4 ura3Δ leu2Δ his3Δ met15Δ |
| LHY4013 | pGFP-RSP5[URA3] ura3Δ leu2Δ his3Δ met15Δ |
| LHY4014 | pGFP-RSP5[URA3] vrp1Δ::kanMX4 ura3Δ leu2Δ his3Δ met15Δ |
| LHY4088 | prsp5 C2Δ [TRP1] pGFP-CPS[URA3] MATα doa4Δ::LEU2 rsp5Δ::HIS3 his3 trp1 ade2 ura3 leu2 lys2 bar1 |
| LHY4227 | pGFP-PHM5[URA3] ura3Δ leu2Δ his3Δ met15Δ |
| LHY4229 | pGFP-PHM5[URA3] tul1Δ::kanMX4 ura3Δ leu2Δ his3Δ met15Δ |
| LHY4281 | pRSP5[TRP1] MATα doa4Δ::LEU2 rsp5Δ::HIS3 his3 trp1 ade2 ura3 leu2 lys2 bar1 |
| LHY4291 | pGFP-CPS[URA3] tul1Δ::kanMX4 ura3Δ leu2Δ his3Δ met15Δ |
| LHY4292 | pRSP5[TRP1] pGFP-CPS[URA3] MATα doa4Δ::LEU2 rsp5Δ::HIS3 his3 trp1 ade2 ura3 leu2 lys2 bar1 |
| LHY4429 | pRSP5[TRP1] pGFP-CPS[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4430 | pRSP5 ww1AxxP [TRP1] pGFP-CPS[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4431 | pRSP5 ww2AxxP [TRP1] pGFP-CPS[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4432 | pRSP5 ww3AxxP [TRP1] pGFP-CPS[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4433 | pRSP5 ww1AxxPww2AxxPww3AxxP [TRP1] pGFP-CPS[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4488 | pGFP-C2[TRP1] his3 trp1 lys2 ura3 leu2 bar1 |
| LHY4489 | pGFP-C2 5K→Q [TRP1] his3 trp1 lys2 ura3 leu2 bar1 |
| LHY4490 | prsp5 5K→Q [TRP1] pGFP-PHM5[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4491 | prsp5 5K→Q [TRP1] pUB-GFP-PHM5[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4492 | pNotI-RSP5[TRP1] pGFP-PHM5[URA3] rsp5Δ::HIS3 his3 leu2 ura3 trp1 bar1 |
| LHY4504 | pGFP-RSP5[TRP1] MATα vps4Δ::kanMX4 rsp5Δ::HIS3 his3 leu2 ura3 trp1 |
| LHY4506 | pGFP-RSP5 5K→Q [TRP1] MATα vps4Δ::kanMX4 rsp5Δ::HIS3 his3 leu2 ura3 trp1 |
| LHY4583 | pGFP-PHM5[URA3] vps23Δ::kanMX4 ura3Δ leu2Δ his3Δ met15Δ |
| LHY4584 | pUB-GFP-PHM5[URA3] vps23Δ::kanMX4 ura3Δ leu2Δ his3Δ met15Δ |
a
All strains are MATa unless otherwise indicated.