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. 2004 Apr 12;165(1):41–52. doi: 10.1083/jcb.200309132

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Ste6-166p requires the Doa10p E3 ubiquitin ligase for degradation. (A and B) Ste6-166p turnover was measured in wild-type, Δhrd1/der3, and Δdoa10 strains by pulse-chase assay as described in Fig. 2, except that cells were pulse labeled for 5 min followed by shorter times of chase. (C) The rate of KWW degradation was determined in wild-type and Δdoa10 cells as described in Fig. 2.