Table II. Plasmids and bacterial strains.
| Strain | Genotype/description | Source |
|---|---|---|
| pMR4937 | FLAG-FUS3 URA3 CEN3 ARS1 AMP R | |
| pMR4938 | FLAG-FUS3Q93G URA3 CEN3 ARS1 AMPR | |
| pMR5049 | FLAG-FUS3Q93G HIS3 CEN3 ARS1 AMPR | |
| pMR3465 | PGAL1-GFP-KAR9 LEU2 CEN4 ARS1 AMP R | |
| p321 | BNI1 disruption construct | Dr. C. Boone, University of Toronto, Toronto, Canada |
| p1955 | 2μ PGAL1-HA-BNI1-GFP URA3 CEN4 ARS1 AMP R | Dr. C. Boone |
| p426S2G | 2μ SPA2-GFP URA3 ARS1 AMP R | Dr. R. Arkowitz, CNRS, Nice, France |
| DSB138 | GPA1 disruption construct | |
| MDB107 | gpa1K21E R22E TRP1 CEN4 ARS1 AMP R | |
| UH7 | ura3::LEU2 KANR AMPR | Dr. F. Cross, The Rockefeller University, New York, NY |
Plasmids were from the laboratories of M.D. Rose, D. Stone, or as indicated.