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. 2004 Sep 13;166(6):901–912. doi: 10.1083/jcb.200404024

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — Actopaxin is phosphorylated in vivo after cell adhesion. Cells were labeled with inorganic ³²P and either held in suspension (lanes 1 and 3) or spread on collagen I–coated dishes for 2 h (lanes 2 and 4). Actopaxin (top) and paxillin (bottom) proteins were immunoprecipitated, resolved on SDS-PAGE, and visualized by autoradiography. WCL, whole cell lysate.