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. 2004 Sep 13;166(6):901–912. doi: 10.1083/jcb.200404024

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Copyright © 2004, The Rockefeller University Press

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Figure 8. — Actopaxin phosphorylation mutants affect Boyden chamber migration. Motility of parental, WT, Quint, and S4/8D cells was evaluated by modified Boyden chamber migration assays. (A) Quantification of random (haptotaxis) motility to collagen (10 µg/ml) or fibronectin (10 µg/ml) demonstrates significant reduction in Quint cell migration as compared with parental and WT cells, whereas S4/8D cells show an increase in migration. (B) Quantification of chemokinesis and chemotaxis in the presence of IGF-II (25 ng/ml) demonstrates a similar trend for each cell line to that seen in the haptotaxis assays. Values are the average of assays performed in triplicate.