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. 2004 Sep 13;166(6):889–900. doi: 10.1083/jcb.200405061

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Copyright © 2004, The Rockefeller University Press

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Figure 6. — Actin and Bem1 function in parallel during cell polarization. (A) Recruitment of Cdc24-GFP by Cdc42^Q61L. HA-tagged Cdc42^Q61L was expressed from the Gal1-promoter in G1-arrested cells that also expressed Cdc24-GFP (RLY1989). Representative DIC and fluorescence images of cells with two buds, one of which must be ectopic. (B) Cdc24-GFP is not recruited to buds of Δbem1 cells expressing HA-Cdc42^Q61L. Representative DIC and fluorescence images of cells with small and medium buds are shown. (A and B) Buds are indicated with arrows. (C) Kinetics of polarization of Cdc24-GFP expressed from the CDC24 promoter in wild-type (RLY1891) or Δbem1 (RLY1961) cells released from G1 arrest. Images show typical caps formed at 35 min after release. Note that the graph for the LatA-treated Δbem1 cells runs along the x axis. (D) Kinetics of polarization of MG-Cdc42 expressed from the CDC42 promoter in wild-type (RLY1950) or Δbem1 (RLY1965) cells released from G1 arrest. Images show typical caps formed at 35 min after release. Note that the graph for the LatA-treated Δbem1 cells runs along the x axis. Error bars correspond to SD. (E) Localization of Cdc24-GFP to multiple caps in Δbem1 cells 25 min after release from G1 arrest. Caps are indicated with arrows. Bars, 5 μm.