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. 2004 Jul 5;166(1):133–143. doi: 10.1083/jcb.200403020

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — Mammary epithelial cells cultured on concentrated EHS ECM form functional acini. (A) Phase-contrast microscopy of primary mouse mammary epithelial cells cultured on concentrated EHS ECM (i) and on EHS ECM diluted (1:1.4) (ii) for 4 d (10X). Bar, 50 μm. (B) Time course of acinus formation from dispersed cells (1–4 d), fluorescence microscopic analysis (staining: DAPI; and Calcein AM and EtBr; 40X). Bar, 20 μm. (C) Fluorescence microscopic analysis of 4 d acini stained with anti–laminin V (i), anti–E-cadherin antibody (ii), phalloidin-FITC (iii), anticleaved caspase 3 (iv; all 40X; bar, 20 μm) and anti–β-casein antibody (v; 20X; bar, 10 μm).