Table I. Percent mtDNA levels in mutants on YEPD vs. YEPGE media as compared with wild type.
| Carbon source | ||
|---|---|---|
| Strain | YEPD | YEPGE |
| Wild type | 100 | 100 |
| mmm2Δ | 16 ± 0.5 | 180 ± 23.7 |
| mmm2Δ mmm1Δ | 38 ± 18.3 | 157 ± 19.5 |
| mmm1Δ | 12 ± 3.9 | 161 ± 13.8 |
Total DNA was isolated from a tetratype tetrad grown in either YEPD or YEPGE, Southern blotted, and probed with 32P-labeled mitochondrial COX2 gene. After normalization to the levels of hybridized nuclear-encoded TIM23 gene, relative amounts of mtDNA (COX2 signal) are compared.