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. 2004 Mar 1;164(5):677–688. doi: 10.1083/jcb.200308012

Table I. Percent mtDNA levels in mutants on YEPD vs. YEPGE media as compared with wild type.

Carbon source
Strain YEPD YEPGE
Wild type 100 100
mmm2Δ 16 ± 0.5 180 ± 23.7
mmm2Δ mmm1Δ 38 ± 18.3 157 ± 19.5
mmm1Δ 12 ± 3.9 161 ± 13.8

Total DNA was isolated from a tetratype tetrad grown in either YEPD or YEPGE, Southern blotted, and probed with 32P-labeled mitochondrial COX2 gene. After normalization to the levels of hybridized nuclear-encoded TIM23 gene, relative amounts of mtDNA (COX2 signal) are compared.