Figure 2.

Role of various regions of vinculin in the control of apoptosis induction. (A) Vinculin mutants: the top line represents full-length vinculin. First mutant: head fragment (1–821). Second mutant: vinculin fragment with talin binding site deleted (243–1066). Third mutant: full-length vinculin containing a Y822F point mutation. Fourth mutant: hinge-tail fragment (811–1066). (B) Expression of these constructs, except for the hinge-tail fragment, was determined by immunoblotting with an antivinculin Ab. (C) Expression of the Flag M2-tagged hinge-tail fragment was determined by immunoblotting with an anti-Flag Ab.