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. 2004 May 10;165(3):371–381. doi: 10.1083/jcb.200308011

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Role of various regions of vinculin in the control of apoptosis induction in a different F9 vin ⁻ ^/ ⁻ clone (γ227). (A) Cell extracts from WT F9 and F9 vin^−/− cells (γ227 clone) stably expressing each construct were analyzed by immunoblotting using anti–caspase-3 Ab 10 h after serum withdrawal. (B) Immunoblotting analysis of cell extracts from WT F9 and F9 vin^−/− cells stably expressing each construct 3 h after disruption of cell–matrix interaction using anti–caspase-3 Ab. The results shown in A and B are representative examples from two independent experiments. (C) Expression of full-length vinculin, head, Δ talin bs, and vinY822F in γ227 clones. (D) Expression of the Flag M2–tagged hinge-tail fragment in γ229 and γ227 clones.