Figure 6.

F9 vin ^−/− and F9 vin ^−/− Y822F cells have an increased paxillin–FAK interaction. (A) Cell extracts from WT F9, F9 vin^−/−, rescue, F9 vin^−/−Y822F, and F9 vin^−/− hinge-tail cells were immunoprecipitated with a mouse antipaxillin Ab, followed by immunoblotting with Ab against FAK. (B) Cell extracts from WT F9, F9 vin^−/−, rescue, F9 vin^−/−Y822F, and F9 vin^−/− hinge-tail cells were immunoprecipitated with a mouse anti-FAK Ab, followed by immunoblotting with Ab against paxillin. The results shown are representative examples from three independent experiments.