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. 2004 May 10;165(3):347–356. doi: 10.1083/jcb.200310015

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — No effect of Bcl-2 overexpression on ER stress-induced cleavage of caspase-4. (a) SK-N-SH cells (left) and HeLa cells (right) stably transfected with the vector or a Bcl-2 expression system were incubated with (+) or without (−) 1 μg/ml tunicamycin for 16 h. Equal amounts of cell lysates were analyzed by Western blotting using anti–caspase-4 antibody (top) and anti–Bcl-2 antibody (bottom). Positions of pro–caspase-4, cleaved caspase-4, and Bcl-2 are indicated. Asterisks show processed caspase-4 as described in Fig. 2 a. (b) The indicated cells were treated with 1 μg/ml tunicamycin for 30 h, stained with Hoechst 33342, and observed under a fluorescence microscope. Bars, 25 μm.