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. 2004 May 10;165(3):323–334. doi: 10.1083/jcb.200311074

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — atToc159 specifically recognizes the transit peptides of photosynthetic preproteins. [³⁵S]atToc159 was incubated with 50 pmol IgG-Sepharose–immobilized pFd-protA in the absence or presence of 0.5 μM pE1α-DHFR_His, pL11-DHFR_His, or pPORA- DHFR_His, or with 50 pmol immobilized pFd-protA_His in the absence or presence of 0.5 μM pFd-protA. The data from triplicate experiments are presented as the percentage of maximal binding of [³⁵S]atToc159 to pFd-protA or pFd-protA_His in the absence of competitor. Error bars represent SEM.