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. 2004 May 10;165(3):323–334. doi: 10.1083/jcb.200311074

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Endogenous soluble atToc159 binds to the transit peptide of preferredoxin. A soluble Arabidopsis protoplast extract containing cytoplasm was applied to Ni-NTA columns containing 75 μg immobilized pFd-protA_His (pFd, lanes 3 and 4) or Fd-protA_His (Fd, lane 5), or Ni-NTA matrix alone (lane 6). Bound proteins were eluted, resolved using SDS-PAGE, and immunoblotted with anti-atToc159 antibodies. Lanes 1 and 2 show 15% of the starting material (S) and unbound fractions (FT) for the pFd-protA_His column. The dividing line indicates grouping of lanes from different parts of the same gel.