Skip to main content
. 2004 May 10;165(3):323–334. doi: 10.1083/jcb.200311074

Figure 7.

Figure 7.

The GTPase domain of atToc159 binds transit peptides selectively. (A) [35S]atToc159, [35S]atToc159G (159G), or [35S]atToc159A (159A) was incubated with 100 pmol Ni-NTA–immobilized pFd-protAHis (lane 2) or Fd-protAHis (lane 3). Bound proteins were eluted, separated by SDS-PAGE and analyzed using a phosphorimager. (B) Quantitation of the data from triplicate experiments including those in A. (C) 100 pmol IgG-Sepharose–immobilized pFd-protA was incubated with [35S]atToc159 in the absence or presence of increasing concentrations of atToc159GHis or CRABPHis. Bound proteins were eluted, resolved by SDS-PAGE, and analyzed using a phosphorimager. (D) Quantitation of the data from replicate experiments including those presented in C. Error bars represent SEM. Lanes labeled IVT in all panels contain 10% of the [35S]atToc159, [35S]atToc159G, or [35S]atToc159A in vitro translation products added to each reaction. Dividing lines in figures indicate grouping of images from different parts of the same gel.