Table III.
Duration of mitosis a in Ptk1 cells treated with nocodazole, anisomycin, or aclarubicin
| Treatment | None | SB203580 |
|---|---|---|
| None | 50 ± 2 (7) | 57 ± 10 (8) |
| Nocodazole | 136 ± 20 (13) | 337 ± 107 (5) |
| Anisomycin (5–7.5 ng/ml) | 46 ± 18 (6) | 40 ± 12 (5) |
| Aclarubicin (1.5–3 μM) | 128 ± 38 (10) | 158 ± 54 (4) |
The duration of mitosis (NEB to anaphase onset) in PtK1 cells treated at 37°C with nocodazole, anisomycin, or aclarubicin, with or without prior treatment with the p38 inhibitor SB203580. The data represents the average ± SD; number of experiments is shown in parentheses.
a
Nuclear envelope breakdown to anaphase onset.