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. 2004 Aug 16;166(4):527–536. doi: 10.1083/jcb.200403102

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Calibration of the YC2 response to stimulation frequency. Trains with different stimulation frequencies ranging from 1 to 50 Hz were applied as indicated to tibialis anterior muscle expressing either YC2 (A and B), split YC2 (B), or a combination of ECFP-CaM and EYFP (B, control), and the response was monitored in situ by two-photon microscopy. (A) Time course of the YFP/CFP ratio values of a representative calibration experiment. (B) Graph showing the mean YFP/CFP ratio as a function of stimulation frequency. Shown are mean YFP/CFP ratio values of the analyzed images for each stimulation frequency. Error bars show ± SD. All data points are derived from at least two different mice and four different fibers.