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. 2004 Aug 16;166(4):493–505. doi: 10.1083/jcb.200403109

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Major and minor satellite-specific marks in interphase and metaphase nuclei. Interphase: (left) HP1α (red) and major and minor DNA (green). (middle) ACA (CENP-A, B, C) is presented in red, major and minor DNA in green. (right) Costaining with ACA serum (red) combined with anti-HP1α antibodies (green). A close-up of selected foci (inset in the merged images). Metaphase: DNA was visualized with DAPI and protein staining in red. Double labeling is shown in merged images. A close-up of a chromosome selected (inset in the merged images). Bars, 5 μm.