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. 2004 Aug 16;166(4):493–505. doi: 10.1083/jcb.200403109

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Copyright © 2004, The Rockefeller University Press

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Figure 6. — Major and minor satellites replication using in situ elongation with BiodU and minor satellite DNA FISH. Sites of DNA synthesis were labeled on MEFs by in situ elongation in the presence of Bio-16-dUTP for 90 min (green), followed by DNA FISH of minor satellites (red). Population 1, nuclei showing a complete staining of the DAPI dense clusters with BiodU. Population 2, nuclei showing a colocalization between BiodU and minor satellites DNA. Bar, 5 μm.