Figure 3.

Dead yeast cells unspecifically bind fluorescein and FITC or FITC-conjugated caspase inhibitor FITC-VAD-FMK. (A) After 24-h incubation at 37°C, the cdc13-1 mutant cells were stained with 10 μM fluorescein or FITC and subjected to flow cytometry analysis. (B) Exponentially growing wild-type cells were either heated to 80°C for 5 min (Dead cells) or maintained at 24°C (Live cells). Cells from both cultures were subsequently labeled with PI and caspase inhibitor FITC-VAD-FMK. Green (FITC) and red (PI) fluorescence of stained cells was measured by flow cytometry.