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Addition of sorbitol to media increases survival of cdc13-1 cells at the restrictive temperature. cdc13-1 and wild-type cells were grown in YPD medium in the presence or absence of 1.2 M sorbitol at 37°C for 24 h. Serial 10-fold dilutions were spotted on a YPD plate and incubated for 3 d at 24°C.
