Table I. Pax7+ve/MyoD−ve satellite cell progeny appear by 72 h in culture.
Pax7 | MyoD | Pax7/ MyoD |
MyoD | Myogenin | MyoD/ Myogenin |
|
---|---|---|---|---|---|---|
Mouse 1 | 19.1% | 66.8% | 14.0% | 16.7% | 4.5% | 78.8% |
(± 1.4) | (± 4.0) | (± 3.5) | (± 4.9) | (± 2.6) | (± 3.6) | |
Mouse 2 | 27.3% | 52.2% | 20.5% | 26.0% | 7.4% | 66.5% |
(± 4.5) | (± 4.4) | (± 2.1) | (± 1.8) | (± 1.5) | (± 3.0) | |
Mean | 23.2% | 59.5% | 17.3% | 21.4% | 6.0% | 72.7% |
Batches of myofibers from the same mouse were coimmunostained for either Pax7 and MyoD (first three columns) or MyoD and myogenin (last three columns). Values are population means from the pooled data from T72 myofibers from each of two age-matched wild-type adult mice. The number of satellite cells in each category is expressed as a mean percentage of the total immunostained cells present on the myofiber (± SEM).