Table II. Most Pax7+ve/MyoD−ve satellite cell progeny arise from Pax7+ve/MyoD+ve parents.
Pax7 | MyoD | Both | Neither | Myogenin | MyoD | Both | Neither | Pax7 | Myogenin | Both | |
---|---|---|---|---|---|---|---|---|---|---|---|
3F-nlacZ-E mouse 3 | |||||||||||
T0 | 3.5 | n/d | n/d | 0.1 | |||||||
(± 0.7) | (± 0.7) | (± 0.1) | |||||||||
T24 | 0.4 | 0 | 5.1 | 0.2 | |||||||
(± 0.2) | (± 0.7) | (± 0.1) | |||||||||
T48 | 0.7 | 0.1 | 9.5 | 0.3 | 0 | 10.4 | 0 | 0.6 | |||
(± 0.3) | (± 0.1) | (± 1.2) | (± 0.1) | (± 1.6) | (± 0.3) | ||||||
T72 | 15.3 | 30.2 | 31.3 | 8.0 | 2.2 | 30.5 | 21.4 | 9.5 | 48.2 | 25.0 | 3.9 |
(± 3.0) | (± 7.5) | (± 5.1) | (± 2.2) | (± 0.8) | (± 5.8) | (± 4.8) | (± 2.8) | (± 5.8) | (± 6.6) | (± 1.2) | |
3F-nlacZ-E mouse 4 | |||||||||||
T0 | 5.3 | n/d | n/d | 0.3 | |||||||
(± 0.7) | (± 0.1) | ||||||||||
T24 | 0.2 | 0 | 5.45 | 0.3 | |||||||
(± 0.1) | (± 0.8) | (± 0.2) | |||||||||
T48 | 0.7 | 0.5 | 19.3 | 0.3 | 0 | 11.5 | 0 | 0.6 | |||
(± 0.2) | (± 0.2) | (± 2.6) | (± 0.2) | (± 2.0) | (± 1.8) | ||||||
T72 | 14.3 | 28.7 | 40.0 | 4.0 | 1.7 | 58.9 | 28.1 | 21.2 | 32.2 | 41.4 | 3.0 |
(± 3.3) | (± 6.3) | (± 6.4) | (± 0.9) | (± 0.8) | (± 3.8) | (± 3.5) | (± 3.5) | (± 5.6) | (± 6.6) | (± 1.0) |
Batches of myofibers from the same mouse were coimmunostained for either Pax7 and MyoD (first four columns), MyoD and myogenin (middle four columns), or Pax7 and myogenin (last three columns) before myonuclear β-galactosidase activity was revealed with X-gal. Values are population means of the number of satellite cells in each category (± SEM) per myofiber isolated from each of two age-matched 3F-nlacZ-E mice. n/d, not determined.