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. 2004 Aug 2;166(3):393–405. doi: 10.1083/jcb.200401093

Figure 2.

Figure 2.

Inhibition by PP2 and Csk of the nectin-induced formation of filopodia and lamellipodia in nectin-1-L cells. (A) Nectin-induced formation of filopodia and lamellipodia. Nectin-1-L or wild-type L cells were cultured on the Nef-3–, IgG-, or PLL-coated coverslips for 30 min and stained for F-actin with rhodamine-phalloidin. Bars in the quantitative analysis represent the number of cells attached on the coverslips per millimeter squared and the percentage of cells with filopodia and/or lamellipodia of the total cells counted (n = 50) and are expressed as means ± SEMs of the three independent experiments. (B) Inhibitory effect of PP2. Nectin-1-L cells were cultured on the Nef-3– or IgG-coated coverslips in the presence of PP2, PP3, or DMSO for 30 min and stained with rhodamine-phalloidin. (C) Inhibitory effect of Csk. Nectin-1-L cells infected with Av1CATcsk or Av1CATlacZ were cultured on the Nef-3–coated coverslips for 30 min and stained with rhodamine-phalloidin and the anti-Csk or the anti–β-galactosidase mAbs, respectively. β-Gal, β-galactosidase. Bars in the quantitative analysis of B and C represent the percentage of cells with filopodia and/or lamellipodia of the total cells counted (n = 50) and are expressed as means ± SEMs of three independent experiments. Bars, 10 μm.