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. 2004 Mar 15;164(6):803–809. doi: 10.1083/jcb.200310148

Figure 2.

Figure 2.

Jasplakinolide addition causes actin clumping, nuclear fragmentation, and increases the level of ROS in yeast cells. (a) KAY339 cells were grown to log phase and half the population was then treated with 2 μM jasplakinolide overnight. Cells were fixed and processed for rhodamine-phalloidin staining to visualize actin, and DAPI was added to allow the organization of DNA to be observed. Bars, 5 μm. (b) Jasplakinolide was added at a range of levels as indicated, and cells were incubated overnight in the presence of H2-DCFDA for analysis by flow cytometry.