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. 2004 Mar 15;164(6):851–862. doi: 10.1083/jcb.200309081

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Copyright © 2004, The Rockefeller University Press

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Figure 9. — Nesca overexpression in NGF-treated PC12 cells results in enhanced levels of activated MAPK. (A) PC12 cell lines stably expressing EGFP and EGFPNesca and (B) EGFP knockdown cell lines (A2, A4, A5, and A6) derived from the EGFPNesca D2 line were serum starved for 24 h followed by the addition of 20 ng/ml NGF for varying lengths of time or for 72 h (EGFPNesca D2 knockdowns). Lysates were analyzed for phosphoMAPK, and the blots were stripped and reprobed with a MAPK antibody to assess protein loading.