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. 2004 Jul 19;166(2):283–295. doi: 10.1083/jcb.200312013

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — Aberrant large protrusion formation in Fak and paxillin knockdown HeLa cells. (A and B) Morphology of siRNA-treated cells replated on collagen at a sparse density and incubated for 3 h (A), and percentages of cells bearing aberrant protrusions (>20 μm in length) are shown (B). Over 70 knockdown cells were counted in each of the four independent experiments. Error bars, SEM. (C) Time-lapse video recording of Fak and paxillin knockdown cells after replating. Numbers represent time in min after the replating. Bars (A and C), 50 μm. (D) Dynamics of the aberrant large protrusion formation. Video images as in C were analyzed and shown as the frequencies of the aberrant protrusion formation (times/cell/h). Error bars, SEM. Abbreviations are the same as in Fig. 1.