Figure 3.

Properties of integrin β1–positive robust FAs in siRNA-treated HeLa cells. siRNA-treated cells were replated onto collagen at a sparse density, incubated for 3 h, and then fixed and subjected to β1 integrin immunostaining (A). Bars, 50 μm. FAs, which were positive for β1 staining and >1 μm in length, were scored for their staining for other proteins (B) as indicated. 4G10; phosphotyrosine. Other abbreviations are the same as in Fig. 1. Over 30 knockdown cells were counted in each of the three independent experiments. Error bars, SEM.