Figure 5.

Effects of Fak and paxillin knockdown on cell motility in BJ cell and of FRNK in HeLa cell. BJ cells were treated with Fak or paxillin siRNAs, or with an irrelevant control siRNA duplex (ctrl) as indicated. (A) 20 μg of each cell lysate was separated by SDS-PAGE and subjected to immunoblot analyses, as indicated. Protein levels of N-cadherin (N-Cad) were also examined. White line indicates that intervening lanes have been spliced out. (B and C) Enhanced migration by knockdown of Fak or paxillin. Cell migration on collagen substrate was monitored (B), and the migration rates were calculated (C) as in Fig. 4, G and H. (D and E) HeLa cells were transfected with empty vector (−) or HA-tagged FRNK cDNA, and were replated onto collagen in the absence of serum. Cell migration was monitored (D), and the migration rates were calculated (E) as in Fig. 4, G and H. Transfected cells were identified by HA staining after video recording. In E, 20 transfected cells were examined in each of three independent experiments. Bars (B and D), 100 μm. Error bars, SEM.