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. 2004 Jul 19;166(2):205–211. doi: 10.1083/jcb.200404150

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — PI(3,4,5)P ₃ biosynthesis requires vps34p and its3p but not fab1p. (A) Wild-type S. pombe, ptn1Δ, vps34Δ ptn1Δ, and fab1Δ ptn1Δ cells were labeled with [³H]inositol. Phosphoinositides were extracted, deacylated, and analyzed by HPLC. Error bars are ± SEM. (B) Wild-type S. pombe, ptn1Δ, its3-1, and its3-1 ptn1Δ cells were labeled and analyzed as in A. In this series of experiments (A and B), we normalized the data in relation to PI because the vps34 and its3-1 mutations resulted in major and reproducible reductions in incorporation of [³H]inositol into the PI phosphate pool.