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. 2004 Jul 19;166(2):205–211. doi: 10.1083/jcb.200404150

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Copyright © 2004, The Rockefeller University Press

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Figure 6. — Phosphoinositide-binding PH domain regulated by ptn1p. (A) Binding of ³⁵S-labeled PH domains to lipids immobilized on a membrane. The Tubby-like and Akt PH domains show specificity for PI(4,5)P₂ and PI(3,4)P₂+PI(3,4,5)P₃, respectively. The 11E3.11C PH domain from S. pombe binds many phosphoinositides, including PI(3,4,5)P₃. (B) Distribution of GFP-11E3.11C PH domain in ptn1Δ cells. The left and right panels show a dividing cell and a growing cell, respectively. Bar, 10 μm. (C) GFP-11E3.11C PH domain shows no clear localization in wild-type cells.