Figure 8.
Live imaging of Borealin-depleted cells reveals severe defects in the partitioning of chromosomes in anaphase. (A–C) β- and γ-tubulin costaining of Borealin-transfected cells (B and C) and a control cell (A). (D) Quantification of multipolar mitotic cells (n = 3; total number of cells scored: control siRNA, 138; Borealin siRNA, 144). (E) Selected frames of a representative live cell imaging experiment performed on mitotic HeLa cells expressing histone H2B-GFP treated with Borealin siRNA. Bar, 5 μm.