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. 2004 Jul 19;166(2):179–191. doi: 10.1083/jcb.200404001

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Copyright © 2004, The Rockefeller University Press

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Figure 9. — Borealin is a substrate for Aurora B kinase in vitro. (A) Kinase activity toward MBP of GST-Aurora B/GST-INCENP/His-Survivin copurified from insect cells using glutathione sepharose beads compared with GST-Aurora B. Black line indicates that intervening lanes have been spliced out. (B) Kinase activity of GST-Aurora B/GST-INCENP/His-Survivin with or without His-Borealin, which was either copurified with the other subunits (left) or added separately in increasing amounts to GST-Aurora B/GST-INCENP/His-Survivin (right). (C) Phosphorylation of GST-Borealin_1–280 and GST-Borealin_142–280, but not GST-Borealin_1–141, by Aurora B. (C') The input of GST-Aurora B/GST-INCENP/His-Survivin was checked by immunoblotting.