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. 2004 Jul 19;166(2):193–203. doi: 10.1083/jcb.200309146

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Bcl-2 inhibits anti-CD3–induced calcium elevation, but not TG-induced calcium elevation. Cytoplasmic calcium elevation induced by anti-CD3 antibody and by TG was measured in parallel in multiple Bcl-2–positive and –negative clones described in Fig. 1. Cells were incubated in ECB containing 1.3 mM calcium until the beginning of recordings when EGTA was added to chelate extracellular calcium. Fluorometry tracings show the transient elevation of cytoplasmic calcium induced by anti-CD3 antibody (A) or by 100 nM TG (B). Histograms summarize the peak cytosolic calcium elevation induced by anti-CD3 antibody (C) or 100 nM TG (D) in multiple experiments (mean ± SEM). F and G summarize multiple experiments (mean ± SEM) in which peak calcium elevation induced by anti-CD3 (E) and by 100 nM TG (F) was measured in WEHI7.2 cells stably expressing Flag-tagged wild-type Bcl-2 and Flag-tagged ER-targeted Bcl-2 or control expression vector (Neo).