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. 2004 Jul 19;166(2):193–203. doi: 10.1083/jcb.200309146

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Bcl-2 inhibits cytoplasmic calcium elevation induced by cell-permeant InsP₃ ester. Cytosolic calcium concentration was measured by single cell calcium imaging using the calcium indicator Fura-2 AM in the presence of 1.3 mM extracellular calcium. (A) Representative calcium traces comparing Bcl-2–negative (N1) and –positive (B27) clones. (B) Summary of peak calcium concentration induced by 25 μM InsP₃ ester in three separate experiments on a total of 42 N1 cells and 31 B27 cells. Symbols represent mean ± SEM and statistical analysis was performed with the Mann-Whitney U Test and confirmed with the Wilcoxon Rank Sum Test.