Figure 5.
Docking-dependent Ca2 + release is stimulated by Sec17/18p. (A) Simulation of Ca2+ release by Sec18p. (B) Sec17/18p promote ongoing Ca2+ release. Standard reactions were initiated, and at 18 min, the microplate was removed from the luminometer, and additional reagents were added: 200 nM of high Sec18p; 150 nM of high Sec17p; and 380 nM of anti-Sec17p or anti-Sec18p IgG. Anti-Sec17/18p antibodies were also added from the reaction start to two samples, as indicated on the plot.