Table I. Antibodies used in the study.
Protein | Antibody | Typea | Reference/Source |
---|---|---|---|
IFT particle proteins | |||
Complex A | |||
IFT139 | 139.1 | M | Cole et al., 1998 |
Complex B | |||
IFT 172 | 172.1 | M | Cole et al., 1998 |
IFT81 | 81.1–4 (pooled) | M | Cole et al., 1998 |
IFT57 | 57.1 | M | Cole et al., 1998 |
FT52 | α-IFT52 | P | Deane et al., 2001 |
IFT74/72 | α-IFT721, α-IFT722 | P | This paper |
Kinesin II motor | |||
FLA10 neck | α-FLA10N | P | Cole et al., 1998 |
FLA10 tail | α-FLA10T | P | Kozminski et al., 1995 |
Cytoplasmic dynein heavy chain | |||
DHC1b | α-DHC1b | P | Pazour et al., 1999 |
Outer arm dynein | |||
Intermediate chain | |||
IC69 | α-IC69 | M | Sigma-Aldrich |
Docking complex | |||
DC83 | α-DC83 | P | Wakabayashi et al., 2001 |
Light chain | |||
LC1 | α-LC1 | P | Benashski et al., 1999 |
LC2 | α-LC2 | P | Patel-King et al., 1997 |
Inner arm dynein | |||
Light chain | |||
Tctex-1 | α-Tctex-1 | P | Harrison et al., 1998 |
Intermediate chain | |||
IC140 | α-IC140 | Yang and Sale, 1998 | |
RSPs | |||
RSP1 | α-RSP1 | P | Williams et al., 1986 |
RSP3 | α-RSP3 | P | Williams et al., 1989 |
Tubulin | |||
Acetylated α-tubulin | M | Sigma-Aldrich | |
β-Tubulin | P | Silflow and Rosenbaum, 1981 | |
Flagellar membrane glycoprotein | |||
FMG-1 | α-FMG-1 | M | Bloodgood et al., 1986 |
Hemagglutinin epitope | 12CA5 | M | Field et al., 1988 |
Ovalbumin | α-Ovalbumin | M | Sigma-Aldrich |
M, monoclonal; P, polyclonal.