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. 2004 May 24;165(4):539–551. doi: 10.1083/jcb.200308141

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — Mapping of α-actinin–binding region on affixin molecule by yeast two-hybrid assay. (A) Isolated positive clones by yeast two-hybrid screening. (B) Specific binding site of α-actinin on affixin molecule. cDNA fragments encoding human l-affixin deletion mutants and full-length α1-actinin were subcloned into pAS2-1 and pGAD10 vectors, respectively. These vectors, obtained clone 94, and pGAD424 full-length ILK were cotransformed into yeast Y187(a) in the indicated combinations, and 5 d later the interactions were examined by β-galactosidase filter assay.