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. 2004 May 24;165(4):517–528. doi: 10.1083/jcb.200403006

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Fat1 colocalized with VASP. (A) PAM212 cells were doubly immunostained for Fat1 (red) and VASP (green). Fat1 colocalized with VASP at lamellipodia. (B) A filopodium of PAM212 doubly immunostained for Fat1 (red) and VASP (green). (C) Fat1 and VASP colocalized at early cell–cell contact sites. (D) Effects of cytochalasin D treatment. PAM212 cells were treated with 150 nM cytochalasin D for 30 min, and were then doubly immunostained for Fat1 (red) and VASP or WAVE (green). The localization of Fat1 or WAVE was not affected, although that of VASP was perturbed. Bars: 15 μm (A), 2.5 μm (B and C), 10 μm (D).