Figure 3.
Affect of ECR3E on αVβ6-mediated latent TGF-β activation and LLC formation. CHO/β6 cells were transduced with empty, ECR3E- or ECR4E-expressing viruses. (A) The transduced cells were co-cultured with TGF-β-reporter TMLCs for 16–24 h before harvesting cell lysates and measuring luciferase activity. Experiments were performed in triplicate and the SDs of a single experiment are given. The errors bars represent the SD of a single experiment that was performed in triplicate. This experiment was repeated multiple times with similar results. (B) The transduced cells were transiently transfected with a TGF-β1 cDNA expression vector and allowed to generate CM for 16–24 h. The media were used for Western blotting. The reactive bands were revealed with an anti-LAP antibody (Vb3A9).