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. 2004 Jun 7;165(5):723–734. doi: 10.1083/jcb.200312172

Figure 7.

Figure 7.

Activation of ECM deposited latent TGF-β. CHO cells stably transfected with ECR3E or LTBP-1S (5 × 104) were plated in a 96-well plate for 48 h before removal with PBS/20 mM EDTA. SW480-ECR3E/β6 or CHO-ECR3E/β6 cells and TMLCs were added to these wells. After 16–24 h, cell lysates were collected and luciferase activity measured. All experimental conditions were performed in triplicate. The SD from a single experiment is shown.