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. 2004 Jun 7;165(5):735–746. doi: 10.1083/jcb.200312170

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Large-scale lateral diffusion measurements by confocal microscopy. (A) Selected images from a confocal FRAP experiment at 37°C of GFP-KRas expressed in COS-7 cells. Bleach box, 4 μm wide. Bar, 10 μm. (B) Kinetics of recovery for 1.4- (circles) versus 4-μm-wide (squares) bleach box. Calculated D and t_1/2 values are indicated. Data shown are for GFP-KRas expressed in COS-7 cells at 37°C. (C) Kinetics of recovery for YFP-GT46 (triangles), YFP-GL-GPI (squares), and GFP-KRas (circles) in COS-7 cells at 37°C using a 4-μm-wide bleach box. Each curve shows the mean ± SD from seven to nine cells from a single experiment. The calculated Ds were as follows: GFP-KRas, 1.01 ± 0.11 μm²/s; YFP-GL-GPI, 0.47 ± 0.07 μm²/s; YFP-GT46, 0.23 ± 0.02 μm²/s.