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. 2004 Jun 7;165(5):641–652. doi: 10.1083/jcb.200403011

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Involvement of the COOH-terminal kinase lobe in Lyn trafficking. (A) Schematic representations of Lyn-HA and its deletion mutants are shown. COS-1 cells were transfected with each of Lyn mutants, and cultured for 48 h. Western blots of equal amounts of Triton X-100 lysates were probed with anti-Lyn or anti-HA. Asterisks indicate the expressed proteins. (B) After transfection with each of Lyn mutants, COS-1 cells were cultured for 48 h. Results (%) of localization of Lyn mutants are the mean ± SD from three independent experiments, as described in Fig. 1 F. (C) Results (%) of localization of Δ 121-324 at 12 and 48 h after transfection into COS-1 cells are shown from a representative and three independent experiments, respectively. PeriN, filled bars; PM, open bars.