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. 2004 Jun 7;165(5):709–721. doi: 10.1083/jcb.200309025

Figure 2.

Figure 2.

Dcx or Lis1 overexpression increases neuronal migration. (A) Retroviral transduction nearly doubles the amount of Dcx or Lis1. Lysates from neurons transduced with RFP-tagged Dcx or Lis1 show bands corresponding to endogenous protein (E) and transduced tagged protein (T). From the ratio of band intensity (T/E) and percentage of transduced cells, transduced protein was ∼85% of the endogenous level. (B–D) Transduction of either Dcx or Lis1 into WT neurons results in an increase in net neuronal migration. The migration distance of each GFP-positive neuron in 12 h is binned. Neurons transduced with either Dcx;GFP or Lis1;GFP display a shift in the bin distribution toward the right. Migration distances are represented in μm along the x axis, and percentage of total neuron number in each bin is represented along the y axis. n = number of neurons measured for each variable. Representative images for each condition are shown. (E) Mean migration distance for neurons overexpressing either Dcx or Lis1 is increased 40–50% compared with GFP alone. **, differs from GFP control; P < 0.01, SNK test. Similar results were obtained on three separate experimental trials. Error bars represent SEM. Bars, 100 μm.