Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Jun 7;165(5):673–683. doi: 10.1083/jcb.200402130

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — D-PLP and GFP-PACT are associated with centrioles. (A) In fixed WT embryos, anti-D-PLP-L antibodies stain centrosomes as a small dot that is located in the center of the PCM (stained here with anti-γ-tubulin antibodies). (B) GFP-PACT colocalizes with D-PLP in these small dots. Note that the intensity of the D-PLP and GFP-PACT staining varies from centrosome to centrosome (A and B, arrows and arrowheads). This is because the D-PLP and GFP-PACT dots are so small that they often cannot all be visualized in the same optical section. (C) In Drosophila oocytes, D-PLP and GFP-PACT colocalize in small dots that appear to be the centrioles (arrows; see text). Both markers also stain the centrioles in the follicle cells that surround the oocyte and nurse cells (arrowheads). GFP-PACT is also present in the oocyte and nurse cell cytoplasm and nuclei, presumably because this fusion protein is expressed at higher levels than the endogenous protein. (D) GTU88* and anti-D-PLP-L antibodies recognize the centrioles in interphase larval neuroblasts. In A–D, DNA is shown in blue in the merged images. (E) In living WT spermatocytes, GFP-PACT is concentrated in the large pair of orthogonally arranged centrioles at the poles of each meiosis I spindle (arrow; inset shows a 3×-magnified view of a centriole pair). The middle panel shows a phase-contrast image, and the right panel a merged image. The panel on the far right shows a schematic representation of the centrioles (green), PCM (red), and microtubules (blue) in a typical meiosis I spindle. (F) In a WT cyst of maturing sperm, GFP-PACT is concentrated in the centrioles/basal bodies located at the base of each sperm flagellum. The panel on the far right shows a schematic representation of the DNA (black), centriole/basal body (green), and microtubules (blue) in a single sperm. Bars, 10 μm.