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. 2004 Jun 21;165(6):843–855. doi: 10.1083/jcb.200401040

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — Identification of Mlc2p. (A) The positions and sequences of the IQ1 and IQ2 motifs in Myo1p. The bold letters indicate generally conserved sequences in IQ motifs of type II myosins. (B) Myo1p-N (the head domain) interacts with Mlc2p by two-hybrid assay. Strain PJ69-4α carrying the bait plasmid pOBD (DBD vector) or pOBD-MYO1-N (DBD-MYO1-N) was crossed with strain PJ69-4a carrying the prey plasmid pOAD alone or carrying one of its derivatives that contain one of the six calmodulin-related genes in S. cerevisiae, MLC2, MLC1, CMD1, FRQ1, CDC31, and CNB1. Diploids from the mating reactions were selected on SC-Trp-Leu and then replica-plated onto SC-His for detecting positive interactions. (C) Alignment of Mlc2p with other myosin II RLCs. The putative phosphorylation site, Ser6, is indicated by an asterisk and the EF hand is marked with a top line. Mlc2p (Scer) from S. cerevisiae; MLC (Scas) from S. castelli (Contig 631.6, http://genome-www4.stanford.edu/cgi-bin/FUNGI/showAlign?locus=YPR188C&source=WashU); Rlc1p (Sp) from S. pombe; and MRLC2 (Hs) from Homo sapiens. Sequences are aligned with MacVector software.