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. 2004 Jun 21;165(6):775–780. doi: 10.1083/jcb.200312030

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — Normal hematopoietic development from caspase-2 ^−/− 9 ^−/− hematopoietic stem cells. Ly5.1 mice that had been lethally irradiated (2 × 5.5Gy) were reconstituted with fetal liver cells and analyzed 10–15 wk later. (A) Staining of thymocytes with antibodies to Ly5.1 (recipient) versus Ly5.2 (donor) cells. (B) Normal cellularities of thymus, lymph nodes (pooled inguinal, axillary, brachial and mesenteric), spleen and bone marrow in animals reconstituted with caspase-2^−/−9^−/−cells. Results represent means ± SD for >3 mice per genotype. (C) Normal composition of the thymus in the reconstituted mice. (D) Normal proportions of B lymphocytes (B220⁺ Thy-1⁻) and T cells (Thy-1⁺ B220⁻) in lymph nodes of the reconstituted mice. Results are representative of >3 mice per genotype.