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. 2004 Jun 21;165(6):775–780. doi: 10.1083/jcb.200312030

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Deficiency of caspases 2 and 9 does not prevent apoptosis in lymphocytes or fibroblasts. To test the sensitivity of lymphocytes to apoptotic stimuli, donor-derived (Ly5.2⁺) (A) lymph node T cells (Thy-1⁺), (B) lymph node B cells (CD45R-B220⁺) and (D) CD4⁺8⁺ thymocytes were purified from the reconstituted mice, or (C) T lymphoblasts were generated by mitogenic activation of spleen cells. (E) Early passage embryonic fibroblasts from E14.5 embryos. In all cases, viability was measured by staining cells with PI and FACS analysis. Results represent means ± SD of >3 mice per genotype.