Figure 5.

Cholesterol depletion affects differently the internalin–E-cadherin and the InlB–HGF-R interactions. Control (−) and cholesterol-depleted (+) cells were incubated for 30 min at 37°C with internalin- or with InlB-coated beads. After three washes cells were fixed and extracellular beads were immunolabeled. The total number of beads (extracellular plus intracellular) associated with the cells, and the number of extracellular beads associated to the cell surface, were assessed by microscopy. Results of at least three independent experiments were expressed relative to control nondepleted cells.